PerkinElmer
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AequoScreen Plasmid

The aequorin plasmid, which expresses the aequorin protein under the control of the CMV promoter, has been developed to convert mammalian cell lines into AequoScreen compatible cell lines.

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ES-002-AC
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Detail Information

The aequorin plasmid, which expresses the aequorin protein under the control of the CMV promoter, has been developed to convert mammalian cell lines into AequoScreen® compatible cell lines. It is a powerful tool which enables the rapid development and analysis of cell lines, already expressing a recombinant or endogenous G-protein coupled receptor of choice, to a luminescence readout. The aequorin plasmid targets cells expressing any protein that modulates intracellular calcium concentration (e.g., GPCRs within the Gq family or GPCRs that couple via a promiscuous G-Protein / chimera).

Specifications

Assay Target Type Cell line
Assay Validated Calcium Luminescence
Product Brand Name AequoScreen
Second Messenger Release Calcium flux
Shipping Condition Dry Ice
Unit Size 10 µg
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Application Note

AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines

Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.

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Flyer

Aequorin FLIPR

Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance

PDF 1 MB

Poster

Running an Aequorin Cell-Based Luminescent Assay on the FLIPR TETRA

We have shown here that signal intensity for the selected catalog aequorin cell lines is strong enough to allow its measurement by the non-luminescence dedicated FLIPR

PDF 270 KB