The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically placed radial groove in the rotor seal which ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.