For Research Use Only. Not for Use in Diagnostic Procedures.
Protein expression can be very easily quantified in High Content Analysis using either fluorescent protein tags or fluorescently labeled antibodies. Expression on the plasma membrane, in the cytosol and in the nucleus can be distinguished by defining the respective cellular regions.
Example for membrane bound marker (left), blue – Hoechst 33342 nuclear dye, green – membrane bound receptor labeled by a fluorescent protein. Region of Interest for protein detection (right).