For Research Use Only. Not for Use in Diagnostic Procedures.
Operetta CLS Application Notes
Live cell imaging has gained importance within drug discovery over recent times, as researchers look for more meaningful insights into cellular behavior and function. In this technical note, we monitor evaporation rates, cell growth and morphology inside the temperature- and CO2-controlled live cell chamber of the Operetta CLS™ high-content analysis system, and compare the results with those obtained using a humidity-controlled cell culture incubator.
Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging.
The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties.
In this application note, we show how a single nuclear stain can enable phenotypic profiling and how phenotypic profiles can be used to distinguish up to seven different cell types, without further staining or phenotypic markers.
Balancing the key factors in HCS imaging - sensitivity, resolution and speed - can be challenging since they cannot be optimized independently: changing one impacts the others. Nevertheless, there is a way to overcome some of the obstacles and here we explain why the choice of the objective lens is critical.