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Bacterial detection probe on the ASK


Overview


XenoLight RediJect Bacterial Detection Probe is a Near Infra-red (NIR) fluorescent agent for in vivo targeting of both Gram Positive and Gram negative bacterial infections. This probe specifically targets the anionic phospholipids of bacterial cell membrane, and so targets Gram negative bacteria slightly more efficiently than Gram positive bacteria. RediJect bacterial detection probe allows detection of deep tissue infections caused by a range of bacteria, including Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium and Escherichia coli.


Products and catalog numbers


ProductCatalog NumberEx/Em wavelength (nm)Validated ExperimentsApplicationsStorage and Stability
XenoLight RediJect Bacterial Detection Probe133398 (20 doses)
133397 (5 dose explorer kit)
750/780In vivo, in vitroBacterial infectionTechnical Data Sheet


Using XenoLight RediJect Bacterial Detection Probe in vivo


ProductRoute of InjectionMouse Dose (25 g)Blood t 1/2Optimal imaging timeRoute of Metabolism/ background tissueIVIS settings
XenoLight RediJect Bacterial Detection ProbeIV100 uL18 h3 -24 hBladderIVIS 745/800

In vivo imaging in mouse model

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Figure 1. In vivo targeting affinity. Four different concentrations (103-106 CFU) of P. aeruginosa and S. aureus were injected on the back of nu/nu mice. 10 nmoles of RediJect Bacterial Detection Probe were injected i.v. and mice were imaged 3 hours post probe injection. The mouse on the left was injected with the probe, the middle mouse got the bacterial injection but no probe, while the mouse on the right received the probe but no bacteria. Top panel are bioluminescent images while the bottom panel shows bacterial detection by the probe using fluorescent imaging. The probe shows better sensitivity with Gram negative bacteria, where 103 CFU are easily detected.

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Figure 2. Non-invasive detection of Streptococcus pneumoniae (Xen10) in a pneumonia model. The brightness of the bacterial detection probe allows for in vivo fluorescence imaging at depth in models like pneumonia. Nu/nu mice were infected with S. pneumoniae strain Xen10 intranasally and infection in the lungs was monitored in real time with bioluminescent imaging (A). 3D reconstructed BLI image was co-registered with micro CT using the Quantum FX micro CT system (B). RediJect Bacterial Detection Probe was administered intravenously and mice were imaged using the transillumination (Trans-Raster 745/800) feature of the IVIS Spectrum. The probe targeting the lung infection can be clearly monitored non-invasively (C).


In vitro imaging


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Figure 3. A) Gram-negative Salmonella typhimurium and Gram-positive S. aureus cells were pre-incubated with the probe for 15 minutes. Cell suspension was triple washed with PBS and cells were then re-suspended in PBS. The yellow circle shows cells not pre-incubated with the probe while the orange circle shows cells pre-incubated with the probe. Plates were imaged using IVIS Spectrum (Ex: 745 nm, Em: 800nm). The chart on the right shows the targeting specificity of the probe. The graph shows more signal coming from Gram-negative than Gram-positive bacteria.


Citations


Search or browse the PerkinElmer Citations Library for references on the Rediject Bacterial Detection probe.