PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

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How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/ASK/LANCE.

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"LANCE TR-FRET" 1-50 of 367 Products & Services

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PerkinElmer

LANCE Ultra ULight™-MBP peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
Price Range : 290.00 EUR - 791.00 EUR

ULight-anti-V5 (LANCE TR-FRET)

Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Price Range : 449.00 EUR - 11400.00 EUR
PerkinElmer

LANCE Eu-anti-C-tag antibody

Europium-labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE ULight or SureLight™ APC-labeled reagents to create LANCE TR-FRET assays.
Price Range : 344.00 EUR - 9700.00 EUR
Protein

p53 (human), lyophilized (LANCE)

Human p53 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra p53 Detection Kit, but can also be ordered separately.
List Price : 1212.00 EUR

ApoE (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ApoE Detection Kit is designed for detection and quantitation of human Apolipoprotein E (ApoE) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 836.00 EUR - 11100.00 EUR
Protein

ANGPTL3 (human), lyophilized (LANCE)

Human angiopoietin-like 3 (ANGPTL3) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human ANGPTL3 Detection Kit, but can also be ordered separately.
List Price : 260.00 EUR

LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 1405.00 EUR - 6500.00 EUR

LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 27 (H3K27ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3195.00 EUR - 8000.00 EUR
LANCE Detection Kit

MMP-8 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human MMP-8 Detection Kit is designed for detection and quantitation of human matrix metalloproteinase 8 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11500.00 EUR

Albumin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Albumin Detection Kit is designed for detection and quantitation of human albumin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11500.00 EUR

ANGPTL3 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ANGPTL3 Detection Kit is designed for detection and quantitation of human angiopoietin-like 3 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11500.00 EUR
LANCE Detection Kit

IL-23A (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-23A Detection Kit is designed for detection and quantitation of human interleukin-23A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 836.00 EUR - 11100.00 EUR
PerkinElmer

LANCE Eu-W1024 Anti-phosphotyrosine (PY20)

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 956.00 EUR - 7900.00 EUR

LANCE Ultra ULight™-JAK-1 (Tyr1023) Peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from amino acids 1015-1027 of human Janus kinase 1 (JAK-1). The ULight-JAK-1 peptide can be phosphorylated at Tyr1023 by JAK3 and several other tyrosine kinases. Peptide sequence: CAGAGAIETDKEYYTVKD
Price Range : 359.00 EUR - 1100.00 EUR
PerkinElmer

Eu-anti-V5 (LANCE TR-FRET)

Europium-labeled anti-V5 antibody, for capturing V5-tagged proteins in LANCE® TR-FRET assays. This Europium-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® ULight or SureLight APC reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Price Range : 334.00 EUR - 9400.00 EUR

LANCE Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody

Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3195.00 EUR - 8000.00 EUR
PerkinElmer

LANCE Eu-W1024 anti-FLAG

Eu-W1024 labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
Price Range : 435.00 EUR - 9800.00 EUR
AlphaLisa Analyte 2

IgG (bovine), lyophilized (LANCE)

Bovine IgG standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Bovine IgG TR-FRET Detection Kit, but can also be ordered separately.
List Price : 252.00 EUR
Protein

ApoA1 (human), lyophilized (LANCE)

Human ApoA1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human ApoA1 Detection Kit, but can also be ordered separately.
List Price : 320.00 EUR

LANCE Ultra Europium-anti-acetyl-Histone H4 Lysine (H4Kac pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 (H4Kac pan). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3195.00 EUR - 8000.00 EUR
LANCE Detection Kit

CHO HCP LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra CHO HCP Detection Kit is designed for detection and quantitation of CHO host cell proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11400.00 EUR
Protein

C-peptide (mouse), lyophilized (LANCE)

Mouse C-peptide standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra C-peptide TR-FRET Detection Kit, but can be ordered separately.

 

List Price : 572.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1/2/3 (Thr308) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT 1/2/3 (Thr308) kits are designed for the detection of phosphorylated AKT1, AKT2, and AKT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR
Protein

BDNF (human), lyophilized (LANCE)

Human brain-derived neurotrophic factor (BDNF) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra BDNF Detection Kit, but can also be ordered separately.
List Price : 320.00 EUR

MBNL1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human MBNL1 Detection Kit is designed for detection and quantitation of human muscleblind-like protein 1 (MBNL1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 2496.00 EUR - 11500.00 EUR

C-peptide (mouse/rat) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse C-peptide Detection Kit is designed for detection and quantitation of Mouse C-peptide in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11400.00 EUR

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM
Price Range : 1321.00 EUR - 4288.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AMPKα (Thr172) LANCE Ultra TR-FRET Detection Kit

LANCE Ultra phospho-AMPKα (Thr172) kits are designed for the detection of phosphorylated AMPKα in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR

LANCE Ultra Europium-anti-phospho-40S Ribosomal Protein S6 (Ser235/236)

Europium-labeled rabbit monoclonal antibody recognizing human 40S ribosomal protein S6 phosphorylated at both Ser235 and Ser236. Sequence motif recognized: RRL(pS)(pS)LRA
Price Range : 655.00 EUR - 2269.00 EUR

LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody

Mouse monoclonal antibody labeled with the LANCE Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated by Ser10. Histone H3 peptides can be phosphorylated by several kinases, including Aurora B. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 1873.00 EUR - 6500.00 EUR
PerkinElmer

LANCE Eu-W1024 anti-FITC

Eu-W1024 labeled anti-FITC antibodies for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 345.00 EUR - 9600.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total ERK 1/2 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra Total ERK 1/2 kits are designed for the detection of total (phosphorylated and non-phosphorylated) ERK1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-ERK detection, and is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR

LANCE® Eu-W1024 labeled anti-GST antibody

Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.
Price Range : 194.00 EUR - 9600.00 EUR
LANCE Detection Kit

TSH (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11500.00 EUR

Leptin (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11500.00 EUR
Protein

COL1A1 (human), lyophilized (LANCE)

Human COL1A1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human COL1A1 TR-FRET Detection Kit, but can also be ordered separately.
List Price : 320.00 EUR

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
Price Range : 1405.00 EUR - 6500.00 EUR

LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
Price Range : 287.00 EUR - 783.00 EUR
LANCE Detection Kit

PER.C6 HCP LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra PER.C6 HCP Detection Kit is designed for detection and quantitation of host cell protein contaminants in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 836.00 EUR - 11100.00 EUR

ULight anti-FITC

ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 463.00 EUR - 11700.00 EUR

IFN-γ (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IFN-γ Detection Kit is designed for detection and quantitation of mouse interferon γ in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11400.00 EUR

CCL2/MCP-1α (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse CCL2/MCP-1α Detection Kit is designed for detection and quantitation of mouse Monocyte Chemoattractant Protein 1α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11400.00 EUR

VEGF (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse VEGF-A Detection Kit is designed for detection and quantitation of human CXCL1/GRO-α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11500.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total STAT3 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total STAT3 kits are designed for the detection of total STAT3 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-STAT3, and is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR

Cortisol LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Cortisol Detection Kit is designed for detection and quantitation of cortisol in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 1685.00 EUR - 11500.00 EUR
Protein

VEGF (human), lyophilized (LANCE)

Human transferrin standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human Transferrin TR-FRET Detection Kit, but can be ordered separately.
List Price : 260.00 EUR
Protein

Fibrinogen (human), lyophilized (LANCE)

Human fibrinogen standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human fibrinogen TR-FRET Detection Kit, but can also be ordered separately.
List Price : 320.00 EUR
PerkinElmer

LANCE Ultra EGFR Positive Control Lysate

Control lysate for LANCE Ultra TR-FRET cellular phospho-EGFR and Total EGFR detection assays.
List Price : 321.00 EUR

LANCE Ultra - KinaSelect™ -TK Kit, 1,000 assay points

LANCE® Ultra TR-FRET tyrosine kinase assay kit.
List Price : 1382.00 EUR
Protein

IGF-1 (rat), lyophilized (LANCE)

Rat IGF-1 (insulin-like growth factor 1) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra rat IGF-1 Detection Kit, but can also be ordered separately.
List Price : 320.00 EUR

"LANCE TR-FRET" 1-50 of 367 Products & Services

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