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Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

  • Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
  • TR-FRET, TRF, and Alpha no-wash technologies for fast and simple measurement of biochemical and cellular enzymatic activity
  • Microfluidic mobility-shift assays (MSA) for radiometric analysis of substrate conversion, kinetics, and mechanism-of-action
  • Multi-mode plate readers for maximal speed and throughput independent of technology platform

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Automation Compatible
  • Yes (231)
Experimental Type
  • In vitro (165)
Format
  • Microplates (180)

"Enzymology" 1-50 of 287 Products and Services

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LabChip EZ Reader

EZ READER, SHIP LEVEL

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
Price Range : 352.00 EUR - 759.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 611.00 EUR - 10900.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-HSP27 (Ser82) Assay Kit

The AlphaScreen® SureFire® p-HSP27 (Ser82) assay is a sandwich immunoassay for quantitative detection of phospho-HSP27 (phosphorylated on Ser82) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-SLP-76 (Ser376) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
Price Range : 508.00 EUR - 29400.00 EUR
SureFire principle

AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit

The AlphaScreen® SureFire® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA Human Total Histone H2A Cell-based Assay Kit

AlphaLISA no-wash assay kit for the detection of total human Histone 2A (H2A) in cell extracts or cells in culture medium.
Price Range : 767.00 EUR - 14300.00 EUR
SureFire principle

AlphaScreen SureFire ELK1 (p-Ser383) Assay Kit

The AlphaScreen® SureFire® p-ELK1 (Ser383) assay is a sandwich immunoassay for quantitative detection of phospho-ELK1 (phosphorylated on Ser383) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR

AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 187.00 EUR - 14300.00 EUR

AlphaLISA Anti-acetyl-Histone H4 Lysine (H4Kac pan) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against acetylated lysine residues on human Histone H4 (H4Kac pan). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1249.00 EUR - 22700.00 EUR
SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser63) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
PerkinElmer

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
Price Range : 258.00 EUR - 1055.00 EUR

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
Price Range : 1348.00 EUR - 6200.00 EUR

LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 1348.00 EUR - 6200.00 EUR
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-EGFR (Tyr1068) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGF Receptor (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SYK Assay Kit

The AlphaLISA® SureFire® Ultra Total SYK assay is a sandwich immunoassay for quantitative detection of SYK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 430.00 EUR - 25000.00 EUR
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA SureFire Ultra kits

Total VEGFR-2 AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ Total VEGFR-2 assay is a sandwich immunoassay for quantitative detection of VEGF receptor 2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 508.00 EUR - 30600.00 EUR

AlphaLISA Anti-mono-methyl-Histone H4 Lysine 20 Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against Histone H4 that is mono-methylated at lysine 20 (H4K20me1). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1249.00 EUR - 22700.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 611.00 EUR - 10900.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-STAT3 (Tyr705) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-STAT3 (Tyr705) kits are designed for the detection of phosphorylated STAT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1275.00 EUR - 5700.00 EUR

LANCE Ultra Europium-anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 mono- and di-methylated at lysine 27 (H3K27me2-1). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3064.00 EUR - 7700.00 EUR
SureFire principle

AlphaScreen SureFire ErbB2 (p-Tyr1221;Tyr1222) Assay Kit

The AlphaScreen® SureFire® p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
PerkinElmer

ProfilerPro Kinase Selectivity Assay Plate Kit 7, for LabChip EZ Reader

Kit consist of matched pairs of 384-well kinase-assay and substrate plates. Each assay plate contains 24 different predispensed frozen kinases. Substrate plates contain the appropriate fluorescent labeled peptides and ATP.
List Price : 1432.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 79 (H3K79me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 79 (H3K79me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 611.00 EUR - 10900.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Ser1106) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR

LANCE Ultra ULight-TK peptide

ULight™-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.
Price Range : 344.00 EUR - 1013.00 EUR

LANCE Ultra Europium-anti-phospho-DNA Topoisomerase 2-alpha (Thr1342)

Europium-labeled mouse monoclonal antibody recognizing human DNA Topoisomerase 2-alpha phosphorylated at Thr1342. Sequence motif recognized: DEK(pT)DDE
Price Range : 1797.00 EUR - 6200.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1/2/3 (Ser473) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1275.00 EUR - 5700.00 EUR
SureFire principle

AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
SureFire principle

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total SLP-76 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.
Price Range : 1275.00 EUR - 5700.00 EUR

LANCE® Eu-W1024 phospho-(Ser) 14-3-3 binding motif antibody, 10 µg

Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for use in LANCE® TR-FRET kinase assays.
List Price : 2990.00 EUR
SureFire principle

AlphaScreen SureFire p-VEGF2 Receptor (Tyr1175) research kit

The AlphaScreen® SureFire® p-VEGFR2 Receptor (p-Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF2 Receptor (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR

AlphaLISA Biotinylated Anti-Histone H3 (C-ter) Antibody

Biotinylated antibody against the C-terminal end of human histone H3, for use in Alpha no-wash epigenetic assays using full-length histone H3 as substrate.
Price Range : 974.00 EUR - 3448.00 EUR
SureFire principle

AlphaScreen SureFire Histone H3 (p-Ser10) Assay Kit

The AlphaScreen® SureFire® p-Histone H3 (Ser10) assay is a sandwich immunoassay for quantitative detection of phospho-Histone H3 (phosphorylated on Ser10) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
SureFire principle

AlphaScreen SureFire STAT3 (p-Tyr705) Assay Kit

The AlphaScreen® SureFire® p-STAT3 (p-Ser705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Ser705) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR
SureFire principle

AlphaScreen SureFire MKK3 (p-Ser189), MKK6 (p-Ser207) Assay Kit

The AlphaScreen® SureFire® p-MKK3 (Ser207) assay is a sandwich immunoassay for quantitative detection of phospho-MKK3 (phosphorylated on Ser207) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR

AlphaLISA Biotin-anti-Histone H4 Antibody

AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H4 in homogeneous AlphaLISA assays.
Price Range : 974.00 EUR - 8700.00 EUR

LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3064.00 EUR - 7600.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-4E-BP1 (Thr37/46) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR

AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1249.00 EUR - 22700.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-Src (Tyr419) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-Src assay is a sandwich immunoassay for quantitative detection of Src (phosphorylated on Tyr419) in cellular lysates using Alpha Technology.

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