PerkinElmer

Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

  • Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
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"Enzymology" 1-50 of 268 Products and Services

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LabChip EZ Reader

EZ READER, SHIP LEVEL

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

ULight™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence : CSERRPHFPQFSYSASGTAR
Price Range : 258.00 EUR - 1055.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
PerkinElmer

ProfilerPro Kinase Selectivity Assay Plate Kit 6, for LabChip EZ Reader

Kit consist of matched pairs of 384-well kinase-assay and substrate plates. Each assay plate contains 24 different predispensed frozen kinases. Substrate plates contain the appropriate fluorescent labeled peptides and ATP.
List Price : 1432.00 EUR

LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide

Glycogen synthase peptide (Sequence: PASVPPSPSLSRHSSPHQ(pS)ED) labeled with ULight™ dye. Synthetic peptide containing residues surrounding Ser641 and Ser657 of human Muscle Glycogen Synthase. This peptide is pre-phosphorylated on Ser657. Target phosphorylation site: Ser641. This peptide substrate can be phosphorylated by a variety of kinases including GSK3α and GSK3β.
Price Range : 435.00 EUR - 759.00 EUR

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
Price Range : 258.00 EUR - 1055.00 EUR
PerkinElmer

LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody

Mouse monoclonal antibody labeled with the LANCE Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated by Ser10. Histone H3 peptides can be phosphorylated by several kinases, including Aurora B. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 1797.00 EUR - 6200.00 EUR
PerkinElmer

ProfilerPro Kinase Peptide Substrate 16, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 16 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 665.00 EUR

LANCE Ultra Europium-anti-phospho-Tyrosine Hydroxylase (Ser40) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from tyrosine hydroxylase and phosphorylated at serine 40. Tyrosine hydroxylase peptides can be phosphorylated by a variety of kinases including PAK2, PKC, CHK1, Aurora A and PKA. Sequence motif recognized: RRQSLIE
Price Range : 1797.00 EUR - 6200.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-Acetyl CoA Carboxylase (Ser79) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-ACC (Ser79) assay is a sandwich immunoassay for quantitative detection of phospho-acetyl CoA carboxylase (phosphorylated on Ser79) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 30600.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-p38 MAPK Kit

The AlphaScreen® SureFire® p-38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Thr1343) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR

LANCE Ultra ULight-TK peptide

ULight™-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.
Price Range : 344.00 EUR - 1013.00 EUR
SureFire principle

AlphaScreen SureFire cJun (p-Ser73) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser73) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
PerkinElmer

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM
Price Range : 1267.00 EUR - 4114.00 EUR

LANCE Ultra ULight™-poly GT

Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.
Price Range : 278.00 EUR - 759.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA Human Total Histone H2A Cell-based Assay Kit

AlphaLISA no-wash assay kit for the detection of total human Histone 2A (H2A) in cell extracts or cells in culture medium.
Price Range : 767.00 EUR - 14300.00 EUR
SureFire principle

AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

The AlphaScreen® SureFire® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR

AlphaLISA Anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 tri-methylated at lysine 27 (H3K27me3). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1249.00 EUR - 22700.00 EUR

AlphaLISA Biotinylated Anti-Histone H3 (C-ter) Antibody

Biotinylated antibody against the C-terminal end of human histone H3, for use in Alpha no-wash epigenetic assays using full-length histone H3 as substrate.
Price Range : 974.00 EUR - 3448.00 EUR

LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
Price Range : 435.00 EUR - 774.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 9 (H3K9me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 9 (H3K9me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 611.00 EUR - 10900.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-EGFR (Tyr1068) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-EGFR (Tyr1068) kits are designed for the detection of EGFR (phosphorylated at residue Y1068) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1275.00 EUR - 5700.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 611.00 EUR - 10900.00 EUR
PerkinElmer

ProfilerPro Kinase Peptide Substrate 18, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 18 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 665.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
PerkinElmer

LANCE Ultra Europium-anti-phospho-Acetyl-CoA Carboxylase (Ser79) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from rat Acetyl-CoA Carboxylase phosphorylated at Ser79. Acetyl-CoA Carboxylase peptides can be phosphorylated by the AMPKα1 kinase. Sequence motif recognized: SSM-pS-GL
Price Range : 1797.00 EUR - 6200.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total AKT1 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total AKT1 kits are designed for the detection of total AKT1 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-AKT1, and is compatible with both adherent and suspension cells.
Price Range : 1275.00 EUR - 5700.00 EUR

AlphaLISA Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is methylated at arginine 2 (H3R2me). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1249.00 EUR - 22700.00 EUR

LANCE Ultra ULight™-Crosstide

Crosstide peptide (sequence: CGSGSGRPRTSSFAEG) labeled with ULight™ dye. Synthetic peptide derived from glycogen synthase kinase-3 alpha (GSK-3α) (Phosphorylation site: Ser21). GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3.
Price Range : 278.00 EUR - 759.00 EUR
SureFire principle

AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit

The AlphaScreen® SureFire® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-4E-BP1 (Thr37/46) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA Eu-N1 Anti-Phosphtyrosine (PT66)

Europium labeled anti-phosphotyrosine PT66 antibody for DELFIA® time-resolved fluorescence (TRF) kinase assays. The PT66 antibody is an affinity purified mouse monoclonal antibody that binds to phosphorylated tyrosine residues.
Price Range : 245.00 EUR - 2858.00 EUR
PerkinElmer

ProfilerPro Kinase Peptide Substrate 27, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 27 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 665.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SYK (Tyr525/526) Assay Kit

The AlphaLISA® SureFire® Ultra p-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (phosphorylated on Tyr525/526) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
PerkinElmer

p53 Acetylated Peptide, for LabChip EZ Reader

Fluorescently-labelled p53 Peptide (Acetylated) for Epigenetic Assays (Enzyme inhibiton, target discovery, lead optimization)
List Price : 544.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Ser1106) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total SLP-76 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.
Price Range : 1275.00 EUR - 5700.00 EUR

LANCE Ultra - KinaSelect™ -TK Kit, 1,000 assay points

LANCE® Ultra TR-FRET tyrosine kinase assay kit.
List Price : 1325.00 EUR
SureFire principle

AlphaScreen SureFire JNK 123 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
Price Range : 352.00 EUR - 759.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-MEK1 (Ser218/222) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.
Price Range : 508.00 EUR - 29400.00 EUR
PerkinElmer

LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP
Price Range : 1348.00 EUR - 6200.00 EUR
PerkinElmer

ProfilerPro Kinase Peptide Substrate 21, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 21 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 665.00 EUR
SureFire principle

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit 

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
PerkinElmer

LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV
Price Range : 1797.00 EUR - 6200.00 EUR
PerkinElmer

LANCE Ultra Europium-anti-phospho-DNA Topoisomerase 2-alpha (Thr1342)

Europium-labeled mouse monoclonal antibody recognizing human DNA Topoisomerase 2-alpha phosphorylated at Thr1342. Sequence motif recognized: DEK(pT)DDE
Price Range : 1797.00 EUR - 6200.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT1 (Ser473) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT1 (Ser473) kits are designed for the detection of AKT1 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1275.00 EUR - 5700.00 EUR

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