PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLisa Analyte 2

Utrophin (human), lyophilized (AlphaLISA)

Human utrophin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human Utrophin Detection Kit, but can also be ordered separately.
List Price : 320.00 EUR
AlphaLisa Analyte 2

MMP-7 (human), lyophilized (AlphaLISA)

Human MMP-7 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human MMP7 Detection Kit, but can be ordered separately.
List Price : 260.00 EUR
AlphaLisa Analyte 2

ANGPTL4 (human), lyophilized (AlphaLISA)

Human ANGPTL4 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human ANGPTL4 Detection Kit, but can also be ordered separately.
List Price : 252.00 EUR

IL-1RA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-1RA enables the detection and quantitation of human Interleukin-1RA in human serum, human plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 482.00 EUR - 10500.00 EUR
Protein

IL-31 (Human) lyophilized (AlphaLISA)

Human IL-31 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA IL-31 Detection Kit, but can be ordered separately.
List Price : 329.00 EUR

Factor VIII (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLaISA® Human Factor VIII Biotin-Free Detection Kit is designed for the quantitative detection of human Factor VIII in buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
Price Range : 496.00 EUR - 10900.00 EUR

IL-13 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 13 Detection Kit is designed for detection and quantitation of human IL-13 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1645.00 EUR - 10900.00 EUR

GAD67 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human GAD67 Detection Kit is designed for detection and quantitation of Glutamic acid decarboxylase 67 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 836.00 EUR - 11100.00 EUR
Protein

TSH (human), lyophilized (AlphaLISA)

Human thyroid stimulating hormone (TSH) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human TSH detection kit, but can also be ordered separately.
List Price : 329.00 EUR
AlphaLISA assay principle

TSH (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TSH enables the quantitative determination of human thyroid stimulating hormone (TSH) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 669.00 EUR - 20100.00 EUR
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA Tb-N1 Streptavidin

Terbium-labeled streptavidin for binding biotinylated biomolecules in DELFIA® time-resolved fluorescence (TRF) assays.
Price Range : 281.00 EUR - 1918.00 EUR
AlphaLISA immunoassay kits

IgA (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 670.00 EUR - 10500.00 EUR
AlphaLisa Analyte 2

MMP-12 (human), lyophilized (AlphaLISA)

Human MMP-12 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human MMP-12 Detection Kit, but can be ordered separately.
List Price : 260.00 EUR

LANCE Ultra Europium anti-phospho-Glycogen Synthase (Ser641)

Europium-labeled rabbit polyclonal antibody recognizing human Muscle Glycogen Synthase phosphorylated at Ser641. Antibody labeled with the LANCE® Eu-W1024-ITC chelate. Sequence motif recognized: PApSVPPSPSL
Price Range : 2183.00 EUR - 6600.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IL2 Detection Kit

The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 657.00 EUR - 10900.00 EUR

CXCL12/SDF-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CXCL12 (SDF-1) enables the quantitative determination of human CXCL12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 468.00 EUR - 10300.00 EUR
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1596.00 EUR - 17200.00 EUR
AlphaLISA immunoassay kits

IgG1 (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG1 antibodies in cell culture media and non-mouse serum.
Price Range : 752.00 EUR - 10500.00 EUR
PerkinElmer

IFN-γ (mouse), lyophilized (LANCE)

Mouse IFNγ standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Mouse IFNγ Detection Kit, but can also be ordered separately.
List Price : 267.00 EUR

LANCE® Eu-W1024 phospho-threonine antibody, 10 µg

Europium-labeled anti-phosphothreonine antibody, for use in LANCE® TR-FRET kinase assays. The antibody is an affinity purified rabbit polyclonal IgG (supplied by New England Biolabs).
List Price : 1139.00 EUR
AlphaLisa Analyte 2

PD-L2 (human), lyophilized (AlphaLISA)

Recombinant human PD-L2 standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human PD-L2 detection kit, but can also be ordered separately.
List Price : 437.00 EUR

Glutathione AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to glutathione. These beads can be used to capture GST-tagged proteins and peptides.
Price Range : 636.00 EUR - 26100.00 EUR
AlphaLISA immunoassay kits

LC3B (human) AlphaLISA Detection Kit

AlphaLISA® no-wash immunoassay kit for detection of human LC3B in buffered solution or cell lysates. The antibodies in the kit detect both LC3B type I and type II. The analyte in this kit consists of recombinant LC3B fused to His-tag at the N-terminus.
Price Range : 3059.00 EUR - 23900.00 EUR
AlphaLISA immunoassay kits

IGF-1 (rat) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for rat IGF-1 enables the quantitative determination of rat insulin-like growth factor 1 (IGF-1) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 649.00 EUR - 19500.00 EUR
AlphaLisa Analyte 2

Amyloid β 1-40 (rodent), lyophilized (AlphaLISA)

Mouse/Rat Amyloid beta (Aß 1-40) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse/Rat Aß 1-40 kit, but can be ordered separately.
List Price : 572.00 EUR
AlphaLISA immunoassay kits

BACE-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BACE-1 enables the quantitative determination of human BACE-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 468.00 EUR - 10300.00 EUR
AlphaLISA immunoassay kits

p62 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for p-62 enables the detection and quantitation of human p62 in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 621.00 EUR - 13600.00 EUR
PerkinElmer

LANCE Ultra cAMP Detection Kit

The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
Price Range : 584.00 EUR - 7600.00 EUR
Alpha donor antibody coated

Anti-Mouse IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 521.00 EUR - 9400.00 EUR
AlphaLISA immunoassay kits

TNFα (mouse) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 698.00 EUR - 10900.00 EUR
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL2 Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 657.00 EUR - 10900.00 EUR
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA® Eu-N1 Anti-6xHis

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in DELFIA® TRF assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6x histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
Price Range : 426.00 EUR - 3978.00 EUR
AlphaLISA immunoassay kits

CD28 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD28 enables the quantitative determination of human CD28 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 649.00 EUR - 15600.00 EUR
AlphaLISA immunoassay kits

MMP-1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Matrix Metalloproteinase-1 (MMP1) Detection Kit is designed for detection and quantitation of human MMP1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP1 and pro-MMP1.
Price Range : 1645.00 EUR - 10900.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1596.00 EUR - 17200.00 EUR
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1767.00 EUR - 18900.00 EUR
AlphaLisa Analyte 2

BACE-1 (human), lyophilized (AlphaLISA)

Human BACE-1 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human BACE-1 Detection Kit, but can also be ordered separately.
List Price : 252.00 EUR
AlphaLISA immunoassay kits

MMP-9 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of human MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.
Price Range : 1645.00 EUR - 10900.00 EUR
LANCE Detection Kit

IFN-γ (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IFN-γ Detection Kit is designed for detection and quantitation of mouse interferon γ in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11400.00 EUR

TRAIL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TNF-related apoptosis-inducing ligand (TRAIL) enables the quantitative determination of human TRAIL in buffered solution and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 811.00 EUR - 11500.00 EUR
LANCE Detection Kit

IgA (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IgA Detection Kit is designed for detection and quantitation of human immunoglobulin A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 861.00 EUR - 11500.00 EUR

Anti-GFP AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaLISA no-wash assays.
Price Range : 636.00 EUR - 26100.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-EGFR (Tyr1068) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-EGFR (Tyr1068) kits are designed for the detection of EGFR (phosphorylated at residue Y1068) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR
AlphaLISA assay principle

IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-23 enables the quantitative determination of human interleukin-23 in human serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 669.00 EUR - 20100.00 EUR

Anti-human IgG (Fc specific) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-human IgG antibody. These beads can be used to capture human IgG antibodies. The antibody conjugated to these beads is Fc-specific, and is a mouse polyclonal antibody.
Price Range : 636.00 EUR - 26100.00 EUR
AlphaLisa Analyte 2

CCL1 (human), lyophilized (AlphaLISA)

Recombinant human CCL1 standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human CCL1 detection kit, but can also be ordered separately.
List Price : 535.00 EUR
AlphaLISA immunoassay kits

Lambda light chain (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human lambda light chain enables the quantitative determination of human λ light chain in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 649.00 EUR - 15600.00 EUR

AlphaScreen Phosphotyrosine (PT66) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 246.00 EUR - 14900.00 EUR

IgG2 (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgG2 in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 725.00 EUR - 11600.00 EUR
AlphaLISA immunoassay kits

IL-17F (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL17F is designed for the quantitative determination of human IL17F in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-17A and with mouse IL17F, bovine IL17F, and rat IL17F. Cross-reactivity with other species has not tested.
Price Range : 669.00 EUR - 20100.00 EUR

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