PerkinElmer

Drug Discovery Reagents

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLisa Analyte 2

Amyloid β 1-42 (human), lyophilized (AlphaLISA)

Human Amyloid beta 1-42 (Aß 1-42) (high specificity) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human Aß 1-42 kit, but can be ordered separately.
List Price : 533.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 594.00 EUR - 10600.00 EUR
Alpha donor antibody coated

Anti-Mouse IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 485.00 EUR - 8800.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 9 (H3K9me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 9 (H3K9me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 594.00 EUR - 10600.00 EUR
AlphaLisa Analyte 2

CCL3 / MIP-1α (human), lyophilized (AlphaLISA)

Human C-C Motif Chemokine 3 / Macrophage Inflammatory Protein-1alpha (CCL3 / MIP-1α) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CCL3/MIP-1α kit, but can be ordered separately.
List Price : 249.00 EUR
AlphaLISA immunoassay kits

Glucagon (human) AlphaLISA Detection Kit

The AlphaLISA® Glucagon Detection Kit is designed for detection and quantitation of glucagon in cell culture media, sera and plasma, in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 701.00 EUR - 9700.00 EUR

IgM (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IgM Detection Kit is designed for detection and quantitation of human IgM in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 1444.00 EUR - 9500.00 EUR
AlphaPlex Samarium Acceptor Beads

Anti-Mouse IgG AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-mouse IgG antibody. These beads are used to capture mouse IgG antibodies (targeting the Fc region).
Price Range : 592.00 EUR - 24300.00 EUR

IFN-α (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IFN-α Detection Kit is designed for detection and quantitation of human interferon α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR

Leptin (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1487.00 EUR - 9900.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated Histone H3 Lysine 9 (H3K9ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 9 (H3K9ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 594.00 EUR - 10600.00 EUR

IL-28B (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-28B Detection Kit is designed for detection and quantitation of human IL-28B in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 899.00 EUR - 12000.00 EUR
AlphaLisa Analyte 2

CCL2 / MCP1 (human), lyophilized (AlphaLISA)

Human Monocyte Chemoattractant Protein-1 (CCL2 / MCP1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CCL2/MCP1 kit, but can be ordered separately.
List Price : 249.00 EUR

Anti-Mouse IgE (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgE. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 569.00 EUR - 23400.00 EUR
LANCE_Fc_receptor

FCGR3A/CD16A (176Phe/F158) (human) LANCE Ultra TR-FRET Binding Kit

This kit is designed for the quantitative determination of Human FCGR3A/CD16a (176Phe/F158) and human Fc fragment binding using a homogeneous (no wash) LANCE Ultra TR-FRET binding assay.
Price Range : 779.00 EUR - 10400.00 EUR
AlphaLISA immunoassay kits

hCG (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human chorionic gonadotropin (hCG) enables the quantitative determination of hCG in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 523.00 EUR - 15700.00 EUR

Anti-Mouse IgM (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgM. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 569.00 EUR - 23400.00 EUR

IgA (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IgA Detection Kit is designed for detection and quantitation of human immunoglobulin A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR
PerkinElmer

AlphaLISA dimethylated-Histone H3 Lysine 79 (H3K79me2) Cellular Detection Kit, bulk orders

This kit is designed for the detection of di-methylated Histone H3 Lysine 79 (H3K79me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

TNFα (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine TNFα enables the detection and quantitation of porcine TNFα in porcine serum, plasma, and, cell lysyate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human and bovine TNFα and no cross reactivity towards porcine IFNγ.
Price Range : 627.00 EUR - 20400.00 EUR
Protein

CXCL1/GRO-α (human), lyophilized (LANCE)

Human CXCL1 (GRO-α) standard, lyophilized, for use in running standard curves in LANCE TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human CXCL1/GRO-α Detection Kit, but can be ordered separately.
List Price : 242.00 EUR
AlphaLisa Analyte 2

Leptin (mouse), lyophilized (AlphaLISA)

Mouse leptin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse leptin kit, but can be ordered separately.
List Price : 242.00 EUR

Frataxin (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human Frataxin (hFXN) in cell and tissue lysates.
Price Range : 700.00 EUR - 10100.00 EUR
Protein

IL-23A (human), lyophilized (LANCE)

Human IL-23A standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IL-23A Detection Kit, but can also be ordered separately.
List Price : 298.00 EUR
AlphaLisa Analyte 2

CXCL1/GROα (human), 0.01 µg, lyophilized (AlphaLISA)

Human CXCL1/GROα standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human CXCL1/GROα Detection Kit, but can be ordered separately.
List Price : 249.00 EUR
AlphaLISA immunoassay kits

IgG1 (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG1 antibodies in cell culture media and non-mouse serum.
Price Range : 700.00 EUR - 9700.00 EUR
AlphaLISA Binding Assay kits

PD-1/PD-L1 (human) AlphaLISA Binding Kit

The AlphaLISA® PD-1/PD-L1 Binding Kit is designed for the detection of binding activity between Human PD-1 and PD-L1, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit PD-1/PD-L1 interaction.
Price Range : 700.00 EUR - 9800.00 EUR
PerkinElmer

Alpha SureFire Ultra Dilution Buffer

Additional buffer for Alpha SureFire Ultra assays.
Price Range : 119.00 EUR - 624.00 EUR
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
Price Range : 623.00 EUR - 18700.00 EUR
Protein

Ghrelin (human), lyophilized (AlphaLISA)

Human ghrelin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human ghrelin detection kit, but can also be ordered separately.
List Price : 298.00 EUR

Glucagon (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Glucagon Detection Kit is designed for detection and quantitation of human glucagon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR

IGF-1 (rat) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Rat IGF-1 Detection Kit is designed for detection and quantitation of rat insulin-like growth factor 1 (IGF-1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 779.00 EUR - 10400.00 EUR

Glutathione AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to glutathione. These beads can be used to capture GST-tagged proteins and peptides.
Price Range : 592.00 EUR - 24300.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total Akt1 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total Akt1 assay is a sandwich immunoassay for quantitative detection of Akt1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 493.00 EUR - 28600.00 EUR

Anti-rat IgG (Fc specific) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-rat IgG antibody. These beads can be used to capture rat IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Price Range : 592.00 EUR - 24300.00 EUR
Protein

Tau (human), lyophilized (LANCE)

Human TAU standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human Tau TR-FRET Detection Kit, but can be ordered separately.
List Price : 242.00 EUR
AlphaLisa Analyte 2

CRP (human), lyophilized (AlphaLISA)

Human C-Reactive Protein (CRP) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CRP kit, but can be ordered separately.
List Price : 249.00 EUR

IgE (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.
Price Range : 700.00 EUR - 9700.00 EUR

Anti-mouse IgG (Fc specific) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-mouse IgG antibody. These beads can be used to capture mouse IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Price Range : 592.00 EUR - 24300.00 EUR
AlphaLisa Analyte 2

PTEN (human), lyophilized (AlphaLISA)

Human PTEN standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human PTEN Detection Kit, but can be ordered separately.
List Price : 249.00 EUR
AlphaLisa Analyte 2

MANF (human), lyophilized (LANCE)

Human MANF standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human MANF TR-FRET Detection Kit, but can also be ordered separately.
List Price : 235.00 EUR

TNFα (mouse) AlphaLISA Detection Kit

The AlphaLISA Mouse TNFα Detection Kit is designed for the quantitative determination of mouse TNFα in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 1532.00 EUR - 10200.00 EUR

MANF (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human MANF Detection Kit is designed for detection and quantitation of human MANF in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 779.00 EUR - 10400.00 EUR
AlphaLisa Analyte 2

Plasma Fibronectin (human), 1 µg, lyophilized (AlphaLISA)

Human plasma fibronectin (hFN) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA® Human Plasma Fibronectin Detection Kit, but can be ordered separately.
List Price : 242.00 EUR
AlphaLisa Analyte 2

IgM (mouse), lyophilized (AlphaLISA)

Mouse IgM lyophilized standard, for use in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgM isotyping kit, but can be ordered separately.
List Price : 239.00 EUR

AlphaLISA Anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Acceptor Beads (for peptide substrate only)

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 di-methylated at lysine 36 (H3K36me2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1213.00 EUR - 22100.00 EUR

LANCE Ultra ULight™-IRS-1 (Tyr983) Peptide

ULight™-labeled synthetic peptide derived from residues 979-989 of mouse Insulin receptor substrate 1 (IRS-1). The ULight-IRS-1 peptide can be phosphorylated at Tyr983 by INSR and JAK2 kinases. Peptide sequence : CKKSRGDYMTMQIG
Price Range : 335.00 EUR - 1024.00 EUR
PerkinElmer

SF UltraPlexTb CaptSure Acc Beads

CaptSure-coated AlphaPlex-545 (Terbium) Acceptor Beads, for multiplexing SureFire assays. These beads are already included in our Alpha SureFire Ultra Multiplex kits, but can be ordered separately as needed.
Price Range : 238.00 EUR - 9800.00 EUR
PerkinElmer

DELFIA® Enhancement Solution, 50 mL

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
List Price : 38.00 EUR
PerkinElmer

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK
Price Range : 251.00 EUR - 5100.00 EUR

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