PerkinElmer

Drug Discovery Reagents

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLisa Analyte 2

Amyloid β 1-40 (rodent), lyophilized (AlphaLISA)

Mouse/Rat Amyloid beta (Aß 1-40) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse/Rat Aß 1-40 kit, but can be ordered separately.
List Price : 533.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-AKT (Thr308) Kit

The AlphaScreen® SureFire® p-Akt1 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 1504.00 EUR - 16200.00 EUR
AlphaLisa Analyte 2

Soybean Agglutinin, liquid (AlphaLISA)

Soybean Agglutinin standard, liquid, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Soybean Agglutinin Detection Kit, but can be ordered separately.
List Price : 249.00 EUR
AlphaPlex Samarium Acceptor Beads

Protein A AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex-645 (Samarium) Acceptor beads conjugated to Protein A. These beads are used to capture immunoglobulins through their heavy chain (Fc region) allowing optimal orientation and antigen detection. Protein A has higher affinity for rabbit, pig, dog and cat IgG.
Price Range : 592.00 EUR - 24300.00 EUR
PerkinElmer

Passive Lysis Solution (PLS) 1X, 100 mL

The Passive Lysis Solution (PLS) is a mammalian cell lysis solution intended to be used to prepare cell lysates for measurement of luciferase activity with the twinlite and sensilite reporter gene assay systems. It is provided in the twinlite and Sensilite kits, but if additional volume of the lysis solution is required, it is also available as a separate item, 100 mL volume per bottle.
List Price : 39.00 EUR

DELFIA Assay Buffer

DELFIA® Assay Buffer is a buffered protein and detergent solution intended for use in DELFIA time-resolved fluorescence (TRF) assays.
Price Range : 33.00 EUR - 591.00 EUR
AlphaLISA immunoassay kits

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 436.00 EUR - 9500.00 EUR

Frataxin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Frataxin Detection Kit is designed for detection and quantitation of human frataxin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR

Anti-rat IgG (Fc specific) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-rat IgG antibody. These beads can be used to capture rat IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Price Range : 592.00 EUR - 24300.00 EUR
AlphaLISA immunoassay kits

CXCL4/PF4 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for CXCL4/PF4 enables the quantitative determination of CXCL4/PF4 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minumum cross-reactivity with mouse CXCL4/PF4 and human CXCL4L1/PF4V.
Price Range : 623.00 EUR - 18700.00 EUR
AlphaLISA immunoassay kits

ApoC3 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ApoC3 enables the quantitative determination of apolipoprotein C3 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 604.00 EUR - 18100.00 EUR
AlphaLISA Buffer

5X AlphaLISA PPI Buffer

General protein-protein interaction assay buffer for AlphaLISA no-wash binding assays. The PPI buffer (5X) has been specially developed for AlphaLISA protein-protein interaction assays.
Price Range : 405.00 EUR - 914.00 EUR
AlphaLISA immunoassay kits

IL-17A (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-17A enables the detection and quantitation of bovine Interleukin 17A (IL17A) in bovine serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 702.00 EUR - 19700.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Price Range : 479.00 EUR - 27800.00 EUR
AlphaPlex Samarium Acceptor Beads

Unconjugated AlphaPlex™ 545 Acceptor Beads

Unconjugated AlphaPlex 645 (Samarium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
Price Range : 1353.00 EUR - 28500.00 EUR
AlphaLisa Analyte 2

GAD67 (Human) lyophilized (AlphaLISA)

Human GAD67 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA GAD67 Detection Kit, but can be ordered separately.
List Price : 299.00 EUR
AlphaLISA immunoassay kits

IL-8 (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human IL8 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-8 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 700.00 EUR - 10100.00 EUR
PerkinElmer

IL-1β (mouse), lyophilized (LANCE)

Mouse IL-1β standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Mouse IL-1β Detection Kit, but can also be ordered separately.
List Price : 249.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA Human Total Histone H4 Cell-based Assay Kit

AlphaLISA no-wash assay kit for the detection of total human Histone H4 in cell extracts or cells in culture medium in a lysate transfer assay.
Price Range : 826.00 EUR - 16200.00 EUR
AlphaLISA Buffer

AlphaLISA NaCl Buffer

Pre-formulated buffer that can be used to dissociate Protein A from IgG in bioprocess samples. This buffer is included in select AlphaLISA immunoassay kits, but can also be purchased to develop your own Alpha assay.
Price Range : 198.00 EUR - 376.00 EUR
AlphaLISA immunoassay kits

IL-6 (monkey) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for monkey IL-6 enables the quantitative determination of cynomolgus monkey interleukin-6 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 604.00 EUR - 18100.00 EUR
AlphaLISA immunoassay kits

FLT-1/VEGFR 1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human VEGFR 1 (FLT-1) enables the quantitative determination of VEGFR 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 436.00 EUR - 9500.00 EUR
AlphaLisa Analyte 2

IL-1β (mouse), lyophilized (AlphaLISA)

Mouse Interleukin-1ß (IL-1ß) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse IL-1ß kit, but can be ordered separately.
List Price : 249.00 EUR
AlphaLisa Analyte 2

Kappa light chain (human), lyophilized (AlphaLISA)

Human kappa light chain standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human Kappa Light Chain Detection Kit, but can also be ordered separately.
List Price : 235.00 EUR
AlphaLISA immunoassay kits

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.
Price Range : 623.00 EUR - 18700.00 EUR
PerkinElmer

SF UltraPlexTb CaptSure Acc Beads

CaptSure-coated AlphaPlex-545 (Terbium) Acceptor Beads, for multiplexing SureFire assays. These beads are already included in our Alpha SureFire Ultra Multiplex kits, but can be ordered separately as needed.
Price Range : 238.00 EUR - 9800.00 EUR
AlphaLISA immunoassay kits

IL-33 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-33 enables the quantitative determination of mouse interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 604.00 EUR - 14500.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human ICAM-1 Detection Kit

The AlphaPlex™-545 (Tb) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).
Price Range : 650.00 EUR - 22900.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total Akt1 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total Akt1 assay is a sandwich immunoassay for quantitative detection of Akt1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 493.00 EUR - 28600.00 EUR
Protein

ApoA1 (human), lyophilized (LANCE)

Human ApoA1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human ApoA1 Detection Kit, but can also be ordered separately.
List Price : 298.00 EUR

Anti-Mouse IgG2a (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG2a. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 569.00 EUR - 23400.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT6 (Tyr641) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (phosphorylated on Tyr641) in cellular lysates using Alpha Technology.
Price Range : 479.00 EUR - 27800.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human TNFα Detection Kit

The AlphaPlex™ 545 (Terbium) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 611.00 EUR - 10100.00 EUR
AlphaLISA immunoassay kits

ANGPTL4 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPTL4 enables the quantitative determination of ANGPTL4 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 436.00 EUR - 9500.00 EUR
AlphaLISA immunoassay kits

Somatostatin (human) AlphaLISA Detection Kit

The AlphaLISA® Somatostatin Detection Kit is designed for detection and quantitation of somatostatin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 700.00 EUR - 10100.00 EUR

IL-28B (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-28B Detection Kit is designed for detection and quantitation of human IL-28B in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 899.00 EUR - 12000.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
List Price : 28600.00 EUR

Anti-acetyl-Histone H3 Lysine 9 (H3K9ac) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 9 (H3K9Ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1262.00 EUR - 23000.00 EUR

AlphaLISA Biotinylated Anti-Histone H3 (C-ter) Antibody

Biotinylated antibody against the C-terminal end of human histone H3, for use in Alpha no-wash epigenetic assays using full-length histone H3 as substrate.
Price Range : 946.00 EUR - 3348.00 EUR
AlphaLISA immunoassay kits

IL-4 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-4 enables the detection and quantitation of bovine interleukin-4 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 449.00 EUR - 9800.00 EUR
Protein

Cardiac Troponin T (human), lyophilized (AlphaLISA)

Human cardiac troponin T standard, for use in running standard curves in AlphaLISA assays.
List Price : 306.00 EUR
AlphaLISA immunoassay kits

Cortisol (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection of human cortisol in buffer or serum.
Price Range : 701.00 EUR - 10100.00 EUR
PerkinElmer

DELFIA® BATDA Labeling Reagent, 50 µL

BATDA reagents for labeling cells in DELFIA time-resolved fluroescence (TRF) assays.
List Price : 158.00 EUR
AlphaLISA immunoassay kits

IL-25 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 604.00 EUR - 14500.00 EUR
AlphaLISA immunoassay kits

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
Price Range : 2934.00 EUR - 22900.00 EUR
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 1650.00 EUR - 17800.00 EUR
Alpha SureFire Ultra Multiplex kits

Alpha SureFire Ultra Multiplex p-AKT (Ser473) (directly coupled) Kit

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).
Price Range : 418.00 EUR - 24300.00 EUR
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
Price Range : 623.00 EUR - 18700.00 EUR
AlphaLisa Analyte 2

CXCL10/IP-10 (mouse), lyophilized (AlphaLISA)

Mouse IP-10 (CXCL10) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IP-10 Detection Kit, but can also be ordered separately.
List Price : 235.00 EUR

Fibronectin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Fibronectin Detection Kit is designed for detection and quantitation of human fibronectin in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR

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