PerkinElmer

Drug Discovery Reagents

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 493.00 EUR - 28600.00 EUR
Protein

FGF23 (Human) lyophilized (AlphaLISA)

Human FGF23 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA FGF23 Detection Kit, but can be ordered separately.
List Price : 242.00 EUR
AlphaLISA immunoassay kits

p62 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for p-62 enables the detection and quantitation of human p62 in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 578.00 EUR - 12600.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Price Range : 479.00 EUR - 27800.00 EUR
AlphaLISA assay principle

FGF23 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FGF23 enables the quantitative determination of human FGF23 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit demonstrated no cross reactivity to FGF21 and very low reactivity to Mouse FGF23.
Price Range : 481.00 EUR - 9800.00 EUR

Lens culinaris agglutinin (LCA) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to LCA (Lens culinaris agglutinin). These beads can be used to capture glycoproteins containing α1,6-linked fucose, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for detection of fucosylated proteins.
Price Range : 574.00 EUR - 23600.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
Price Range : 493.00 EUR - 28600.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total ERK 1/2 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 493.00 EUR - 28600.00 EUR
AlphaLISA immunoassay kits

BCL-2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for BCL-2 enables the detection and quantitation of human BCL-2 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). This kit is not cross reactive with mouse Bcl-2.
Price Range : 627.00 EUR - 20400.00 EUR

Anti-Chicken IgY AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-chicken IgY antibody. The antibody is a rabbit polyclonal antibody that is Fc-specific. These beads can be used to capture chicken IgY antibodies for AlphaLISA no-wash assays.
Price Range : 592.00 EUR - 24300.00 EUR

Frataxin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Frataxin Detection Kit is designed for detection and quantitation of human frataxin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR
AlphaLisa Analyte 2

BACE-1 (human), lyophilized (AlphaLISA)

Human BACE-1 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human BACE-1 Detection Kit, but can also be ordered separately.
List Price : 235.00 EUR
AlphaLISA immunoassay kits

Insulin-like Growth Factor 2 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Insulin-like Growth Factor 2 (IGF2) Detection Kit is designed for detection and quantitation of human IGF2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1532.00 EUR - 10200.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 9200.00 EUR
Protein

Frataxin (human), lyophilized (LANCE)

Human Frataxin standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human Frataxin TR-FRET Detection Kit, but can be ordered separately.
List Price : 242.00 EUR
AlphaLISA immunoassay kits

BDNF (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of Brain Derived Neurotrophic Factor (BDNF) in serum, cell culture supernatants, and other samples.
Price Range : 701.00 EUR - 10100.00 EUR

Anti-GST AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-GST antibody. These beads can be used to capture GST-tagged proteins and peptides in AlphaLISA no-wash assays.
Price Range : 592.00 EUR - 24300.00 EUR
AlphaLisa Analyte 2

α-tubulin standard, liquid (AlphaLISA)

Human α-tubulin standard, liquid, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA α-tubulin Detection Kit, but can be ordered separately.
List Price : 429.00 EUR
AlphaLISA immunoassay kits

Fibronectin (human plasma) AlphaLISA Detection Kit

The AlphaLISA® Human Plasma Fibronectin Detection Kit is designed for detection and quantitation of human plasma fibronectin (hFN) in human serum, plasma, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 449.00 EUR - 9800.00 EUR
AlphaLisa Analyte 2

cAMP standard, solid (AlphaLISA)

cAMP standard, solid, for use in running standard curves in AlphaLISA cAMP assays. This standard is already provided in the AlphaLISA cAMP kit, but can be ordered separately.
List Price : 242.00 EUR
AlphaLISA immunoassay kits

TGF-β1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human TGF-β1 Detection Kit is designed for detection and quantitation of human TGF-β1 (hTGF-β1) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with human hTGF-β2 and hTGF-β3.
Price Range : 700.00 EUR - 10100.00 EUR
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA® Eu-N1 Anti-GST

Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in DELFIA® TRF assays.
Price Range : 226.00 EUR - 2774.00 EUR
AlphaLisa Analyte 2

ß-NGF (human), lyophilized (AlphaLISA)

Human Beta-Nerve Growth Factor (β-NGF) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human β-NGF kit, but can be ordered separately.
List Price : 249.00 EUR

CXCL9/MIG (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CXCL9/MIG Detection Kit is designed for detection and quantitation of human CXCL9/MIG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR
Protein

CCL3/MIP1α (human), lyophilized (LANCE)

Human CCL3 (MIP1α) standard, lyophilized, for use in running standard curves in LANCE TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human CCL3/MIP1α Detection Kit, but can be ordered separately.
List Price : 242.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 1665.00 EUR

Glucagon (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Glucagon Detection Kit is designed for detection and quantitation of human glucagon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR
AlphaLISA immunoassay kits

α-tubulin AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for α-tubulin enables the detection and quantitation of human α-tubulin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 702.00 EUR - 19700.00 EUR
AlphaLisa Analyte 2

ErbB2 / HER2 (human), lyophilized (AlphaLISA)

Human epidermal growth factor receptor 2 (ErbB-2, NEU, or HER2) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. The standard consists of the extracellular domain of human HER2 fused to the Fc region of human IgG1. This standard is already provided in the AlphaLISA human ErbB2 kit, but can be ordered separately.
List Price : 249.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.
Price Range : 493.00 EUR - 28600.00 EUR
AlphaLISA immunoassay kits

Cardiac Troponin I (human) AlphaLISA Detection Kit

The AlphaLISA® Human cardiac Troponin I Detection Kit is designed for detection and quantitation of human cardiac Troponin I in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 2934.00 EUR - 22900.00 EUR
AlphaLISA immunoassay kits

IL-21 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-21 enables the quantitative determination of human interleukin-12 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 604.00 EUR - 14500.00 EUR
AlphaLISA immunoassay kits

AlphaLISA Human Programmed Cell Death Protein 1 (PD-1) Detection Kit

The AlphaLISA® Human PD-1 Detection Kit is designed for detection and quantitation of Human Programmed Cell Death Protein 1 (PD-1) in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 650.00 EUR - 10100.00 EUR
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA® Eu-N1 Anti-HA

Europium-labeled anti-HA antibody for binding HA-tagged proteins in DELFIA® time-resolved fluorescence (TRF) assays. The anti-HA antibody is a purified IgG2b subclass of mouse monoclonal antibody. It recognizes the epitope sequence (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.
Price Range : 537.00 EUR - 5100.00 EUR
AlphaLISA immunoassay kits

Factor VIII (human) AlphaLISA Detection Kit

The AlphaLISA® Human Factor VIII Detection Kit is designed for detection and quantitation of Human Factor VIII in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 650.00 EUR - 10100.00 EUR

TNFα (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human TNFα Detection Kit is designed for detection and quantitation of human TNFα in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR

LANCE® Eu-W1024 Anti-Rabbit IgG

Europium-labeled anti-rabbit IgG, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 891.00 EUR - 7800.00 EUR

Transferrin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Transferrin Detection Kit is designed for detection and quantitation of human transferrin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR
AlphaLisa Analyte 2

MMP-7 (human), lyophilized (AlphaLISA)

Human MMP-7 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human MMP7 Detection Kit, but can be ordered separately.
List Price : 242.00 EUR
AlphaLISA immunoassay kits

Amyloid β 1-42 (high specificity) (human) AlphaLISA Detection Kit

The AlphaLISA® Human Amyloid beta 1-42 (Aß 1-42) (high specificity) Detection Kit is designed for detection and quantitation of human Aß 1-42 in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 2934.00 EUR - 22900.00 EUR

AlphaScreen GST Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 181.00 EUR - 13900.00 EUR
Protein

ANGPT2 (human), lyophilized (AlphaLISA)

Human Angiopoietin 2 (ANGPT2) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human ANGPT2 Detection Kit, but can also be ordered separately.
List Price : 298.00 EUR
AlphaPlex Samarium Acceptor Beads

Anti-Human IgG AlphaPlex 645 (Sm) Acceptor beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-human IgG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 592.00 EUR - 24300.00 EUR
alphascreen surefire total

AlphaScreen SureFire Total p38 MAPK Assay Kit

The AlphaScreen® SureFire® total p38 MAPK assay is a sandwich immunoassay for quantitative detection of p38 MAPK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 1650.00 EUR - 17800.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 28600.00 EUR
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology.
Price Range : 1504.00 EUR - 16200.00 EUR
AlphaLISA assay principle

BMP-9 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BMP-9 enables the quantitative determination of human Bone Morphogenetic Protein 9 (BMP-9) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 604.00 EUR - 18100.00 EUR
AlphaLisa Analyte 2

PAI-1 (human), lyophilized (AlphaLISA)

Human Plasminogen Activator Inhibitor-1 (PAI-1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. The analyte consists of the active glycosylated human PAI-1. This standard is already provided in the AlphaLISA human PAI-1 kit, but can be ordered separately.
List Price : 533.00 EUR

Anti-Histone H3 (C-term) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads designed to capture Histone H3 in a homogeneous AlphaLISA assay for DOT1L. Use in conjunction with biotinylated anti-H3K79me2 (AL148).
Price Range : 1213.00 EUR - 22100.00 EUR
AlphaLISA assay principle

ANGPT1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPT1 enables the quantitative determination of human Angiopoietin 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 604.00 EUR - 18100.00 EUR

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