Streamline Your Analysis of CRISPR-Cas9 Genome Editing
CRISPR-Cas9 genome editing is a transformative technology that allows researchers to edit sections of DNA with a high degree of precision. The CRISPR system can be exploited by researchers to target specific genetic loci, enabling them to edit DNA at precise locations, permanently modifying cells and organisms. This genome editing can be used to create animal models and cell models of diseases of interest, facilitating new avenues of research.
The LabChip® GX Touch™ Electrophoresis System is an invaluable tool to simplify and accelerate CRISPR-Cas9 experiments and analysis. The LabChip GX Touch Electrophoresis System:
Assesses DNA fragments at concentrations as low as 2 pg/µL
Analyzes up to 384 samples in as little as 28 seconds per sample
Identifies slight differences in sample size with high degrees of sensitivity and resolution
Improves reproducibility and precision of analysis
Cited in over 100 CRISPR studies
For research use only. Not for use in diagnostic procedures.
Quantitation and visualization of CRISPR homoduplexes and heteroduplex digestion products
In a recent CRISPR-Cas9 study, the LabChip GX Touch instrument was used to automate a workflow that detects the on-target genome editing events in CRISPR-Cas9-manipulated cells. The LabChip 12K DNA assay accelerated the detection of the on-target efficiency of editing events by quantitating and visualizing both intact homoduplexes and heteroduplex digestion products.
Improved size distribution analysis of input DNA and CRISPR modified products
The LabChip GX Touch system is also used to simplify other CRISPR-Cas9 applications by automating the capillary electrophoresis used to assess sample size distribution of input DNA and CRISPR modified products. In a recent article published in Nature Communications, researchers at Stanford University developed a novel STR-seq protocol to identify short tandem repeats (STRs) in genetic material. In their research, the team, led by Dr. Hanlee P. Ji, relied on the LabChip GX Touch system’s automated capillary electrophoresis capabilities to assess the sample size distribution of the input DNA and confirm the product size of the gRNAs developed to target the STRs.
Shin, G. et al. CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis. Nat. Commun. 8, 14291 doi: 10.1038/ncomms14291 (2017).
DNA and RNA quantitation and sizing can be done in seconds using automated capillary electrophoresis separation. The LabChip® GX Touch™ nucleic acid analyzer’s microfluidic technology generates reproducible, high-resolution data and is optimal for
NGS library preparation (smear and fragment analysis) and quality control
RNA and DNA fragment analysis (including cell-free (cfDNA), FFPE, liquid biopsy, and PCR-free samples)
Quantitation and qualification for CRISPR fragment analysis