PerkinElmer

AlphaLISA Binding Kits

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The interactions and binding of proteins are implicated in a large number of biological processes. The need for an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size. A bead-based proximity assay, Alpha Technology offers the possibility to assay many biological targets, including enzymes, receptor-ligand interactions, low affinity interactions, second messenger levels, DNA, RNA, proteins, peptides, sugars and small molecules.

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

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Assay Target
  • 4-1BB (1)
  • 4-1BBL (1)
  • CD80 (1)
  • CD86 (1)
  • CTLA4 (2)
  • EGF (1)
  • EGFR (1)
  • FCGR1 (1)
  • FCGR2A (2)
  • FCGR2B (1)
  • FCGR3A (2)
  • HLA-DRA (1)
  • LAG3 (1)
  • PD-1 (2)
  • PD-L1 (2)
  • PDGF-BB (2)
  • PDGFR (2)
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Target Species
  • Human (10)
  • Mouse (1)
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Application
  • Protein Analysis & Detection (8)
  • Protein-protein interactions (16)
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Therapeutic Area
  • Biologics/Bioprocess (6)
  • Cancer (9)
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Assay points(200-50,000)
  • 100 (4)
  • 500 (17)
  • 5000 (17)
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Assay Target Class
  • Fc-receptor (6)
  • Protein (9)
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Experimental Type
  • In vitro (4)
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Format
  • Microplates (4)
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Pipetting Arms
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"AlphaLISA Binding Kits" 1-17 of 17 Products & Services

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AlphaLISA immunoassay kits
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FCGR2B/CD32b AlphaLISA Binding Kit

AL3080C , AL3080F
This kit is designed for the detection of binding between FCGR2B (CD32b) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding.
Price Range : 2297.00 EUR - 11500.00 EUR
Assay principle for LANCE TR-FRET binding assay
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CTLA4-CD80 AlphaLISA Binding Kit

AL3046C , AL3046F
This kit is designed for the detection of binding activity between Human CTLA-4 and CD80, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding to CTLA-4/CD80.
Price Range : 1947.00 EUR - 11900.00 EUR
AlphaLISA immunoassay kits
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PD-1/PD-L1 (human) AlphaLISA Binding Kit

AL356C , AL356F , AL356HV
The AlphaLISA® PD-1/PD-L1 Binding Kit is designed for the detection of binding activity between Human PD-1 and PD-L1, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit PD-1/PD-L1 interaction.
Price Range : 752.00 EUR - 10500.00 EUR
AlphaLISA immunoassay kits
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EGF/EGFR AlphaLISA Binding Kit

AL366C , AL366F , AL366HV
The AlphaLISA® EGF/EGFR Binding Kit is designed for the detection of binding activity between Human EGF and EGFR, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit the EGF/EGFR interaction.
Price Range : 752.00 EUR - 10500.00 EUR
Alpha Fc receptor bind
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FCGR3A/CD16a (176Val/V158) AlphaLISA Binding Kit

AL348HV , AL348F , AL348C
This kit is designed for the detection of binding activity between human FCGR3A (176Val) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 752.00 EUR - 11500.00 EUR
AlphaLISA FCGR3A/CD16A Detection Kit, 5000
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FCGR3A/CD16A (176Phe/F158) (human) AlphaLISA Detection Kit

AL347F , AL347C , AL347HV
This kit is designed for the detection of binding activity between human FCGR3A (176Phe) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 631.00 EUR - 11500.00 EUR
Assay principle for AlphaLISA binding assay
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4-1BB/4-1BBL (human) AlphaLISA Binding Kit

AL3113F , AL3113C
This kit is designed to assess inhibitors of human 4-1BB and human 4-1BBL binding, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to human 4-1BB/4-1BBL.
Price Range : 2313.00 EUR - 11600.00 EUR
AlphaLISA immunoassay kits
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FcγR3B (CD16b) AlphaLISA Binding Kit

AL3094C , AL3094F
The AlphaLISA FCGR3B (CD16b) binding kit is designed for the detection of binding between FcγR3B (CD16b) and human IgG Fc fragment using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics using a competition assay format.
Price Range : 2296.00 EUR - 11500.00 EUR
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FcRn (human) AlphaLISA Binding Kit

AL3095F , AL3095C
The AlphaLISA human FcRn binding kit is designed for the detection of binding between FcRn and human IgG using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics using a competition assay format.
Price Range : 2296.00 EUR - 11500.00 EUR
Assay principle for LANCE TR-FRET binding assay
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CTLA4-CD86 AlphaLISA Binding Kit

AL3047F , AL3047C
This kit is designed for the detection of binding activity between Human CTLA-4 and CD86, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding to CTLA-4/CD86.
Price Range : 1947.00 EUR - 11900.00 EUR
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FcγR2A/CD32a (167R) AlphaLISA Binding Kit

AL3087F , AL3087C
This kit is designed for the detection of binding between FCGR2A (CD32a) (167R) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding.
Price Range : 2297.00 EUR - 11500.00 EUR
AlphaLISA binding assay
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PDGF-BB/PDGFRα (human) AlphaLISA Binding Kit

AL3064C , AL3064F
This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRα, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRα.
Price Range : 1890.00 EUR - 15100.00 EUR
AlphaLISA binding assay
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PDGF-BB/PDGFRβ (human) AlphaLISA Binding Kit

AL3065C , AL3065F
This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRβ, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRβ.
Price Range : 1890.00 EUR - 15100.00 EUR
AlphaLISA immunoassay kits
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FCGR1/CD64 AlphaLISA Binding Kit

AL3081C , AL3081F
This kit is designed for the detection of binding between FCGR1 (CD64) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 2297.00 EUR - 11500.00 EUR
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FcγR2A/CD32a (167H) AlphaLISA Binding Kit

AL3086F , AL3086C
This kit is designed for the detection of binding between FCGR2A (CD32a) (167H) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding.
Price Range : 2297.00 EUR - 11500.00 EUR
Assay principle for AlphaLISA mouse PD-1/PD-L1 binding assay
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PD-1/PD-L1 (mouse) AlphaLISA Binding Kit

AL580F , AL580C
The AlphaLISA® mouse PD-1/PD-L1 binding assay enables development and characterization of small and large molecule anti-mouse PD-1 or PD-L1 reagents for in vitro and in vivo studies, testing of other putative binding ligands such as PD-L2 or characterization of ligand subregions for their ability to block binding, and determination of mouse cross-reactivity of anti-human PD-1 or PD-L1 reagents using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 2297.00 EUR - 11500.00 EUR
AlphaLISA binding assay
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HLA-DRA/LAG3 (human) AlphaLISA Binding Kit

AL3066F , AL3066C
This kit is designed for the detection of binding activity between Human HLA-DRA and LAG3, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to HLA-DRA and LAG3.
Price Range : 1890.00 EUR - 15100.00 EUR

"AlphaLISA Binding Kits" 1-17 of 17 Products & Services