PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 966 Products and Services

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AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA immunoassay kits

IgM (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgM in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 669.00 EUR - 10700.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Ser727) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Ser727) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR
Alpha_Donor_bead_1

AlphaScreen Streptavidin Donor beads

Alpha Donor beads conjugated to streptavidin.
Price Range : 374.00 EUR - 10500.00 EUR
SureFire principle

AlphaScreen SureFire NFkB (p-Ser536) Assay Kit

The AlphaScreen® SureFire® p-NF-κB p65 (p-Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NF-κB p65 (phosphorylated on Ser536) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR
AlphaLISA SureFire Ultra kits

Total p38 MAPK AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra Total p38 MAPK assay is a sandwich immunoassay for quantitative detection of p38 MAPK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay for phosphorylation studies.
Price Range : 508.00 EUR - 30600.00 EUR

Neprilysin (human) AlphaLISA Detection Kit

The AlphaLISA® Neprilysin Detection Kit is designed for detection and quantitation of human neprilysin (NEP, CD10) in cell culture media, serum, and CSF (cerebrospinal fluid) in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 721.00 EUR - 10400.00 EUR
alphascreen surefire total

AlphaScreen SureFire JNK 123 Total Assay Kit

The AlphaScreen® SureFire® total JNK1/2/3 assay is a sandwich immunoassay for quantitative detection of JNK1/2/3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 14900.00 EUR
AlphaPlex-545-antibody-bead2

Anti-HA AlphaPlex 545 (Tb) Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-HA (hemaglutinin) antibody. These beads can be used to capture HA-tagged proteins and peptides.
Price Range : 610.00 EUR - 25100.00 EUR
AlphaLISA assay principle

Osteoprotegerin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for total osteoprotegerin enables the quantitative determination of human osteoprotegerin in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 641.00 EUR - 19200.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR

Galectin-9 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human galectin-9 enables the quantitative determination of human galectin-9 (Gal-9) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 778.00 EUR - 14900.00 EUR

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
Price Range : 3023.00 EUR - 23600.00 EUR
AlphaLISA immunoassay kits

TNFα (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine TNFα enables the detection and quantitation of porcine TNFα in porcine serum, plasma, and, cell lysyate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human and bovine TNFα and no cross reactivity towards porcine IFNγ.
Price Range : 646.00 EUR - 21000.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD3 (Ser423/425) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD3 (Ser423/425) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR
Alpha_nickel_Donor

Nickel Chelate Alpha Donor beads

Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.
Price Range : 500.00 EUR - 9100.00 EUR
PerkinElmer

AlphaLISA SureFire Ultra Total p38 MAPK Lysate

Extra control lysates for the AlphaLISA® SureFire® Ultra total p38 MAPK assay.
List Price : 22.00 EUR
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR

Protein G AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to Protein G. These beads can be used to capture antibodies that associate with Protein G.
Price Range : 610.00 EUR - 25000.00 EUR
Alpha_Donor_bead_1
Price Range : 58.00 EUR - 4956.00 EUR
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR
AlphaLISA immunoassay kits

IFN-α (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon-alpha (IFN-α) Detection Kit is designed for detection and quantitation of human IFN-α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit consists of the IFN-α 2b subtype.
Price Range : 1578.00 EUR - 10500.00 EUR
Alpha Fc receptor bind

FCGR3A/CD16a (176Val/V158) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Val) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 721.00 EUR - 11000.00 EUR
SureFire principle

AlphaScreen SureFire IKKa (p-Ser176/Ser180) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR
PerkinElmer

SureFire Ultra Activation Buffer C (50X)

Extra activation buffer for the AlphaLISA SureFire Ultra pSTAT4 assay. This buffer is already included in the AlphaLISA SureFire Ultra pSTAT4 kit, but can also be ordered separately.
Price Range : 122.00 EUR - 642.00 EUR
AlphaLisa Analyte 2

SHP-2 (human), lyophilized (AlphaLISA)

Human SHP-2 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human SHP-2 Detection Kit, but can also be ordered separately.
List Price : 407.00 EUR

MBNL1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human muscleblind-like protein 1 (MBNL1) enables the quantitative determination of human MBNL1 in buffered solution and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1813.00 EUR - 14500.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-GSK-3β (Ser9) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-GSK-3β (Ser9) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR

TIM-3 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TIM-3 enables the quantitative determination of human T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 623.00 EUR - 14900.00 EUR
AlphaLisa Analyte 2

TSLP (human), lyophilized (AlphaLISA)

Human TSLP standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human TSLP Detection Kit, but can also be ordered separately.
List Price : 306.00 EUR
AlphaLisa Analyte 2

D-dimer (human), lyophilized (AlphaLISA)

Human D-dimer standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human D-dimer kit, but can be ordered separately.
List Price : 549.00 EUR
AlphaLISA immunoassay kits

IFNα (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IFNα is designed for the quantitative determination of mouse interferon-alpha (IFN-α) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 641.00 EUR - 19200.00 EUR

AlphaScreen General IgG (Protein A) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.
Price Range : 93.00 EUR - 14300.00 EUR
Alpha_Protein_Donor_bead

Protein A Alpha Donor beads

Alpha Donor beads conjugated to Protein A. This bead can be used to capture antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 500.00 EUR - 9100.00 EUR
PerkinElmer
Price Range : 291.00 EUR - 11900.00 EUR

Protein A AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to Protein A. These beads can be used to capture antibodies that associate with Protein A.
Price Range : 610.00 EUR - 25000.00 EUR
PerkinElmer

Alpha SureFire Ultra Lysis Buffer (5X)

Additional buffer for Alpha SureFire Ultra assays.
Price Range : 122.00 EUR - 643.00 EUR

Anti-C-tag AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-C-tag antibody. These beads can be used to capture C-tag proteins, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays.
Price Range : 610.00 EUR - 25000.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Total Cofilin Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two targets in each well of an assay plate. This kit can be used in combination with the AlphaLISA SureFire Ultra phospho-cofilin assay to normalize to levels of total cofilin.
Price Range : 323.00 EUR - 19500.00 EUR
AlphaLISA SureFire Ultra kits

Total STAT5 AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ Total STAT5 assay is a sandwich immunoassay for quantitative detection of STAT5 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 508.00 EUR - 29400.00 EUR
Alpha donor antibody coated

Anti-Mouse IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 500.00 EUR - 9100.00 EUR
AlphaLISA immunoassay kits

IL-17F (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL17F is designed for the quantitative determination of human IL17F in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-17A and with mouse IL17F, bovine IL17F, and rat IL17F. Cross-reactivity with other species has not tested.
Price Range : 641.00 EUR - 19200.00 EUR
AlphaLisa Analyte 2

Leptin (mouse), lyophilized (AlphaLISA)

Mouse leptin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse leptin kit, but can be ordered separately.
List Price : 249.00 EUR

Anti-Mouse IgG2b (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG2b. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 586.00 EUR - 24100.00 EUR

AlphaScreen FITC (Fluorescein) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FITC conjugated AlphaScreen Acceptor beads. 
Price Range : 199.00 EUR - 14300.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-MEK1 (Ser218/222) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLisa Analyte 2

TAU (human), lyophilized (AlphaLISA)

Human Microtubule-associated Protein Tau (TAU) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human TAU kit, but can be ordered separately.
List Price : 549.00 EUR
AlphaLISA immunoassay kits

Amyloid β 1-40 (human) AlphLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human Amyloid β 1-40 (Aβ 1-40) in cerebrospinal fluid (CSF), cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 3081.00 EUR - 23600.00 EUR

IL-2 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-2 (IL-2) Detection Kit is designed for detection and quantitation of mouse IL-2 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1578.00 EUR - 10500.00 EUR
AlphaLISA immunoassay kits

FCGR3A/CD16A (176Phe/F158) (human) AlphaLISA Detection Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Phe) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 605.00 EUR - 11000.00 EUR

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