PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1003 Products & Services

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Il-12 (p70) (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 12 (p70) Detection Kit is designed for detection and quantitation of human IL-12 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1645.00 EUR - 10900.00 EUR

AlphaLISA Biotin-anti-Histone H4 Antibody

AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H4 in homogeneous AlphaLISA assays.
Price Range : 1015.00 EUR - 9100.00 EUR

Anti-H3K36me2 AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-H3K36me2 antibody. These beads can be used for AlphaLISA epigenetic assays using histone or nucleosome substrates.
Price Range : 1302.00 EUR - 23700.00 EUR
Alpha donor antibody coated

Anti-GFP Alpha Donor Beads

Alpha Donor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaScreen, AlphaLISA, and AlphaPlex no-wash assays.
Price Range : 662.00 EUR - 9500.00 EUR
AlphaLISA immunoassay kits

IL-13 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 13 Detection Kit is designed for detection and quantitation of human IL-13 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1645.00 EUR - 10900.00 EUR
AlphaPlex Samarium Acceptor Beads

Anti-Mouse IgG AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-mouse IgG antibody. These beads are used to capture mouse IgG antibodies (targeting the Fc region).
Price Range : 636.00 EUR - 26100.00 EUR
Assay principle for AlphaLISA mouse PD-1/PD-L1 binding assay

PD-1/PD-L1 (mouse) AlphaLISA Binding Kit

The AlphaLISA® mouse PD-1/PD-L1 binding assay enables development and characterization of small and large molecule anti-mouse PD-1 or PD-L1 reagents for in vitro and in vivo studies, testing of other putative binding ligands such as PD-L2 or characterization of ligand subregions for their ability to block binding, and determination of mouse cross-reactivity of anti-human PD-1 or PD-L1 reagents using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 2297.00 EUR - 11500.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

The AlphaScreen® SureFire® p-β-catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-β-catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1596.00 EUR - 17200.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IL2 Detection Kit

The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 657.00 EUR - 10900.00 EUR
AlphaLISA immunoassay kits

HIV p24 AlphaLISA Biotin-Free Detection Kit

The AlphaLISA HIV p24 Biotin-Free Detection Kit is designed for the quantitative detection of HIV p24 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 752.00 EUR - 10900.00 EUR
AlphaLisa Analyte 2

Lambda light chain (human), lyophilized (AlphaLISA)

Human lambda light chain standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human lambda light chain Detection Kit, but can also be ordered separately.
List Price : 320.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA Total Histone H4 Cellular Detection Kit

The AlphaLISA® Total Histone H4 Cellular Detection Kit is designed for the detection of total human Histone H4 (modified and unmodified) in cell extracts or cells in culture medium, using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 2152.00 EUR - 12300.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Price Range : 523.00 EUR - 30300.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SLP-76 (Ser376) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.
Price Range : 333.00 EUR - 20000.00 EUR

AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1302.00 EUR - 23700.00 EUR
AlphaLisa Analyte 2

TNFα (cynomolgus), lyophilized (AlphaLISA)

Cynomolgus TNFα standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Cynomolgus TNFα Detection Kit, but can also be ordered separately.
List Price : 252.00 EUR

Anti-FITC AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to mouse monoclonal anti-FITC antibody. These beads can be used to capture FITC-tagged and fluorescein-labeled biomolecules for AlphaLISA no-wash assays.
Price Range : 636.00 EUR - 26100.00 EUR
AlphaLisa Analyte 2

IL-11 (human), lyophilized (AlphaLISA)

Human Interleukin 11 (IL-11) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human IL-11 kit, but can be ordered separately.
List Price : 267.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 637.00 EUR - 11400.00 EUR
AlphaLISA immunoassay kits

Fibrinogen (human) AlphaLISA Detection Kit

The AlphaLISA® Human Fibrinogen Detection Kit is designed for detection and quantitation of Human fibrinogen in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 482.00 EUR - 10900.00 EUR

Anti-human IgG (Fc specific) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-human IgG antibody. These beads can be used to capture human IgG antibodies. The antibody conjugated to these beads is Fc-specific, and is a mouse polyclonal antibody.
Price Range : 636.00 EUR - 26100.00 EUR

Total Ghrelin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human total ghrelin enables the quantitative determination of total ghrelin in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 649.00 EUR - 19500.00 EUR
AlphaLISA immunoassay kits

Monocyte Chemoattractant Protein-1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Monocyte Chemoattractant Protein-1 (CCL2 / MCP1) Detection Kit is designed for detection and quantitation of human CCL2/MCP1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1645.00 EUR - 10900.00 EUR
AlphaLisa Analyte 2

TREM2 (human), liquid (AlphaLISA)

Recombinant human TREM2 standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human TREM2 detection kit, but can also be ordered separately.
List Price : 788.00 EUR
AlphaLISA immunoassay kits

TNFα (cynomolgus) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for cynomolgus TNFα enables the quantitative determination of cynomolgus tumor necrosis factor alpha in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 468.00 EUR - 10300.00 EUR
Fc-fragment-conjugated Alpha Donor beads

Fc-fragment (human) conjugated Alpha Donor Beads

Alpha Donor beads conjugated to human IgG Fc fragment. These beads can be used to capture Fc binding proteins and receptors.
Price Range : 524.00 EUR - 9500.00 EUR
AlphaLISA SureFire Ultra kits

Total eIF2α AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ total eIF2α assay is a sandwich immunoassay for quantitative detection of eIF2α (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-eIF2α assay.
Price Range : 523.00 EUR - 31500.00 EUR
AlphaLisa Analyte 2

Protein A, lyophilized (AlphaLISA)

Protein A lyophilized standard, to be used in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Residual A Research Kits, but can be ordered separately.
List Price : 267.00 EUR
AlphaLISA immunoassay kits

Leptin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Leptin Detection Kit is designed for detection and quantitation of human leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Due to the presence of leptin binding proteins in serum or other matrices, an acid-dissociation step might be required before performing the AlphaLISA assay.
Price Range : 1645.00 EUR - 10900.00 EUR
PerkinElmer

AlphaLISA SureFire Ultra Total Cofilin Lysate

Extra control lysates for the AlphaLISA® SureFire® Ultra total cofilin assay.
List Price : 23.00 EUR
Alpha_nickel_Donor

Nickel Chelate Alpha Donor beads

Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.
Price Range : 521.00 EUR - 9400.00 EUR
AlphaLISA immunoassay kits

IL-12 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IL-12 enables the quantitative determination of porcine interleukin-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 649.00 EUR - 19500.00 EUR

TNFα (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine TNFα in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 698.00 EUR - 11100.00 EUR
AlphaLISA immunoassay kits

GDNF (human) AlphaLISA Detection kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human GDNF (glial cell-derived neurotrophic factor) in serum, plasma, cell culture supernatants, and other samples.
Price Range : 698.00 EUR - 10900.00 EUR
AlphaLISA immunoassay kits

IFN-α (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon-alpha (IFN-α) Detection Kit is designed for detection and quantitation of human IFN-α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit consists of the IFN-α 2b subtype.
Price Range : 1645.00 EUR - 10900.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1767.00 EUR - 18900.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 79 (H3K79me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 79 (H3K79me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 637.00 EUR - 11400.00 EUR
AlphaLisa Analyte 2

IL-13 (bovine), lyophilized (AlphaLISA)

Bovine IL-13 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Bovine IL-13 Detection Kit, but can also be ordered separately.
List Price : 252.00 EUR
AlphaLISA immunoassay kits

C-Reactive Protein (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-Reactive Protein (CRP) Detection Kit is designed for detection and quantitation of human CRP in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1645.00 EUR - 10900.00 EUR
AlphaLisa Analyte 2

IL-1ß (rat), lyophilized (AlphaLISA)

Rat Interleukin 1ß (IL-1ß) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Rat IL-1ß kit, but can be ordered separately.
List Price : 267.00 EUR
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IP10 Detection Kit

The AlphaPlex Samarium (Sm) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 745.00 EUR - 10900.00 EUR
AlphaLisa Analyte 2

Myeloperoxidase (human), lyophilized (AlphaLISA)

Human Myeloperoxidase (MPO) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human MPO kit, but can be ordered separately.
List Price : 572.00 EUR
AlphaLISA immunoassay kits

Ghrelin (active) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for active human ghrelin enables the quantitative determination of active ghrelin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 649.00 EUR - 19500.00 EUR
AlphaLisa Analyte 2

MMP9 (human), lyophilized (AlphaLISA)

Human Matrix Metalloproteinase-9 (MMP9) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human MMP9 kit, but can be ordered separately.
List Price : 267.00 EUR
AlphaLISA assay principle

IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-23 enables the quantitative determination of human interleukin-23 in human serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 669.00 EUR - 20100.00 EUR
AlphaLISA immunoassay kits

Albumin (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA® Human Albumin Biotin-Free Detection Kit is designed for the quantitative detection of human albumin in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
Price Range : 482.00 EUR - 10500.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-SLP-76 (Ser376) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
Price Range : 523.00 EUR - 30300.00 EUR
AlphaLisa Analyte 2

NT-proBNP (human), lyophilized (AlphaLISA)

Recombinant human N-terminal prohormone of brain natriuretic peptide (NT-proBNP) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human NT-proBNP detection kit, but can also be ordered separately.
List Price : 787.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human/Rat IL22 Detection kit

The AlphaPlex 545 (Terbium) Human / Rat IL22 Detection Kit is designed for the quantitative determination of human or rat IL22 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 698.00 EUR - 10900.00 EUR
AlphaLISA immunoassay kits

IL-6 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-6 (IL-6) Detection Kit is designed for detection and quantitation of mouse IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit is a superior alternative to a traditional mouse IL-6 ELISA kit due to its simple and fast protocol, wide dynamic range, and high sensitivity.
Price Range : 1645.00 EUR - 10900.00 EUR

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