PerkinElmer

Alpha Reagents

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Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 906 Products and Services

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AlphaLISA immunoassay kits

IL-6 (monkey) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for monkey IL-6 enables the quantitative determination of cynomolgus monkey interleukin-6 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 623.00 EUR - 18700.00 EUR
SureFire principle

AlphaScreen SureFire S6RP (p-Ser235;Ser236) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser235/236) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser235/236) in cellular lysates using Alpha Technology.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLisa Analyte 2

IL-12 (porcine), lyophilized (AlphaLISA)

Porcine IL-12 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA porcine IL-12 Detection Kit, but can also be ordered separately.
List Price : 306.00 EUR
alphascreen surefire total

AlphaScreen SureFire p53 Total Assay Kit

The AlphaScreen® SureFire® total p53 assay is a sandwich immunoassay for quantitative detection of p53 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 424.00 EUR - 16700.00 EUR
AlphaLISA immunoassay kits

IFN-γ (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IFN-γ enables the detection and quantitation of porcine interferon-gamma in serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human, bovine, ovine, or equine IFNγ. Cross reactivity with other species has not been tested.
Price Range : 646.00 EUR - 21000.00 EUR
AlphaLISA immunoassay kits

TNFα (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine TNFα in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 669.00 EUR - 10700.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Thr1343) in cellular lysates using Alpha Technology.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaPlex Samarium Acceptor Beads

Unconjugated AlphaPlex™ 545 Acceptor Beads

Unconjugated AlphaPlex 645 (Samarium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
Price Range : 1393.00 EUR - 29300.00 EUR
AlphaLisa Analyte 2

CXCL10/IP-10 (mouse), lyophilized (AlphaLISA)

Mouse IP-10 (CXCL10) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IP-10 Detection Kit, but can also be ordered separately.
List Price : 242.00 EUR
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLisa Analyte 2

IgM (bovine), lyophilized (AlphaLISA)

Bovine IgM standard, 10 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA bovine IgM kits, but can also be ordered separately.
List Price : 347.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human ICAM-1 Detection Kit

The AlphaPlex™-545 (Tb) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).
Price Range : 669.00 EUR - 23600.00 EUR
alphascreen surefire total

AlphaScreen SureFire Total STAT3 Assay Kit

The AlphaScreen® SureFire® total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 510.00 EUR - 18400.00 EUR

AlphaScreen HA (Hemagglutinin) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-HA (hemagglutinin) conjugated AlphaScreen Acceptor beads. 
Price Range : 190.00 EUR - 14300.00 EUR
Alpha-Unconjugated-Donor-bead

AlphaScreen Unconjugated Donor Beads

Alpha Unconjugated Donor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own Alpha Donor beads for use in Alpha no-wash assays.
Price Range : 374.00 EUR - 10500.00 EUR
AlphaLISA immunoassay kits

Fibronectin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human fibronectin enables the quantitative determination of human fibronectin in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 778.00 EUR - 14900.00 EUR
AlphaLisa Analyte 2

IFNβ (porcine), lyophilized (AlphaLISA)

Porcine interferon β (IFNβ) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IFNβ Detection Kit, but can also be ordered separately.
List Price : 732.00 EUR

Anti-DIG AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a mouse monoclonal anti-Dig (digoxin) antibody. These beads can be used to capture Dig-labeled proteins, peptides, and other biomolecules to create AlphaLISA no-wash assays.
Price Range : 610.00 EUR - 25000.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.
Price Range : 1716.00 EUR - 18400.00 EUR
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA assay principle

Total Ghrelin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human total ghrelin enables the quantitative determination of total ghrelin in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 623.00 EUR - 18700.00 EUR
AlphaLISA immunoassay kits

IgM (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgM in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 669.00 EUR - 10700.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex™ 545 IL-6 Detection Kit 

The AlphaPlex™ 545 (Terbium) IL-6 Detection Kit is designed for the quantitative determination of human IL6 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 630.00 EUR - 10400.00 EUR
alphascreen surefire total

AlphaScreen SureFire Total Topo Iia Assay Kit

The AlphaScreen® SureFire® total Topoisomerase II assay is a sandwich immunoassay for quantitative detection of Topoisomerase II (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-JNK1/2/3 (Thr183/Tyr185) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA immunoassay kits

CXCL4/PF4 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for CXCL4/PF4 enables the quantitative determination of CXCL4/PF4 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minumum cross-reactivity with mouse CXCL4/PF4 and human CXCL4L1/PF4V.
Price Range : 641.00 EUR - 19200.00 EUR

AlphaScreen Phosphotyrosine (PT66) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 236.00 EUR - 14300.00 EUR
alphascreen surefire total

Total ERK (monoclonal) AlphaScreen SureFire Assay Kit

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. This assay performs similarly to the TGRTES SureFire kit, but contains a monoclonal antibody for ERK detection.
Price Range : 510.00 EUR - 18400.00 EUR
AlphaScreen Acceptor Beads

AlphaScreen Unconjugated Acceptor Beads

Unconjugated AlphaScreen® Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.
Price Range : 374.00 EUR - 10500.00 EUR
AlphaLISA immunoassay kits

IgG4 (human) isotyping AlphaLISA Detection Kit

The AlphaLISA Human IgG4 Detection Kit is designed for detection, quantitation, and isotyping of human IgG4 in cell culture medium and non-human serum, using a homogeneous (no wash steps, no separation steps) assay. The assay shows negligible cross-reactivity with other human IgG isotypes and monkey IgG.
Price Range : 643.00 EUR - 10000.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Tyr701) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Tyr701) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR
AlphaLISA immunoassay kits

PDGF (BB dimer) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PDGF (BB homodimer) enables the quantitative determination of human platelet-derived growth factor (PDGF) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 623.00 EUR - 14900.00 EUR
AlphaLISA immunoassay kits

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 449.00 EUR - 9800.00 EUR
AlphaLISA immunoassay kits

IgG4 (human) Pharmacokinetic AlphaLISA Detection Kit

The AlphaLISA® Human IgG4 Pharmacokinetic Detection Kit is designed for the quantitative determination of human IgG4 in monkey serum and other samples, using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 1532.00 EUR - 10200.00 EUR
AlphaLISA assay principle

FGF23 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FGF23 enables the quantitative determination of human FGF23 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit demonstrated no cross reactivity to FGF21 and very low reactivity to Mouse FGF23.
Price Range : 496.00 EUR - 10100.00 EUR
AlphaLISA immunoassay kits

IgE (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.
Price Range : 721.00 EUR - 10000.00 EUR

Anti-c-myc AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-c-myc antibody. These beads can be used to capture c-myc-tagged proteins and peptides in AlphaLISA no-wash assays.
Price Range : 610.00 EUR - 25000.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-STAT1 (Tyr701) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA immunoassay kits

Amyloid β 1-40 (human) AlphLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human Amyloid β 1-40 (Aβ 1-40) in cerebrospinal fluid (CSF), cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 3081.00 EUR - 23600.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA immunoassay kits

EGF (human) AlphaLISA Detection Kit

The AlphaLISA® EGF Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor (EGF) in cell culture media, serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 669.00 EUR - 10400.00 EUR
AlphaLISA immunoassay kits

IgA (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgA in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 669.00 EUR - 10700.00 EUR

Anti-Mouse IgA AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgA. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 586.00 EUR - 24100.00 EUR
AlphaLISA SureFire Ultra kits

Total STAT5 AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ Total STAT5 assay is a sandwich immunoassay for quantitative detection of STAT5 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 508.00 EUR - 29400.00 EUR
AlphaLISA immunoassay kits

Residual Protein A AlphaLISA Detection Kit

The AlphaLISA Residual Protein A Detection Kit is designed for the quantitative detection of Protein A contaminants in buffered solution using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 788.00 EUR - 6600.00 EUR
AlphaLisa Analyte 2

Amyloid β 1-42 (human), lyophilized (AlphaLISA)

Human Amyloid β 1-42, lyophilized, 0.3 µg. Amyloid β 1-40 is included in the AlphaLISA® human Amyloid β 1-42 kits, but can also be ordered separately as a standalone item. One vial contains an amount of human Amyloid β 1-40 sufficient for performing 10 standard curves.
List Price : 256.00 EUR
AlphaLISA immunoassay kits

Heme oxygenase 1 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 1 (HO-1) enables the quantitative determination of human HO-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 449.00 EUR - 9800.00 EUR

AlphaScreen General IgG (Protein A) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.
Price Range : 93.00 EUR - 14300.00 EUR
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human ICAM-1 Detection Kit

The AlphaPlex™-645 (Sm) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).
Price Range : 669.00 EUR - 23600.00 EUR

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